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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed

 

Search Results for: imaging conjunctival vessels (selections from 738 results)

 

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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9194771

 

http://atvb.ahajournals.org/cgi/content/full/17/6/1178

 

Arterioscler Thromb Vasc Biol. 1997 Jun;17(6):1178-84.  Related Articles, Links 

 

 

Vitamin C reduces cholesterol-induced microcirculatory changes in rabbits.

 

Freyschuss A, Xiu RJ, Zhang J, Ying X, Diczfalusy U, Jogestrand T, Henriksson P, Bjorkhem I.

 

Department of Medicine, Karolinska Institute, Huddinge University Hospital, Sweden.

 

The microcirculation was studied for 10 weeks in untreated rabbits (n = 12) and in rabbits treated with vitamin C in their drinking water (0.5 g/d; n = 6), a 1% cholesterol diet (n = 12), or a combination of the two treatments (n = 11). The studies were performed by direct intravital microscopic imaging of the conjunctiva of both eyes to evaluate blood flow velocity, microvessel diameter, and microhemorheologic conditions. As we reported previously, changes occurred in all of the aforementioned variables as a consequence of cholesterol feeding. After 3 and 6 weeks of feeding, there was a marked and significant (P < .0001) decrease in blood flow velocity in third-order arterioles, which was accompanied by stasis and erythrocyte aggregation in the smaller conjunctival vessels. When cholesterol treatment was combined with vitamin C, blood flow was almost identical to that of controls and significantly (P < .0001) higher than that of rabbits treated with cholesterol alone. All other changes were also significantly reduced by the addition of vitamin C treatment to the cholesterol diet. Cholesterol-treated rabbits developed macroscopic arterial lesions that were not significantly reduced by vitamin C treatment. Neither circulating oxysterol levels nor atheromas were reduced by vitamin C treatment, which also had no significant effect on lipid or circulating vitamin E levels. We have previously shown that the lipid-soluble antioxidant BHT is able to prevent both cholesterol-induced microcirculatory changes and the development of arterial lesions in rabbits. This phenomenon is compatible with a critical oxidation step occurring in the lipid phase that is common to both processes. The finding that microcirculatory changes can be prevented by a water-soluble antioxidant is compatible with a role for water-soluble oxidants in this context. The possibility is discussed that vitamin C might also be important for the microcirculation in humans.

 

PMID: 9194771 [PubMed - indexed for MEDLINE]

 

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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12090638

 

Ophthalmic Physiol Opt. 2002 May;22(3):234-43.  Related Articles, Links 

 

 

Optimal green (red-free) digital imaging of conjunctival vasculature.

 

Owen CG, Ellis TJ, Rudnicka AR, Woodward EG.

 

Department of Public Health Sciences, St George's Hospital Medical School, London, UK. c.owen@sghms.ac.uk

 

AIMS/BACKGROUND: Green illumination is commonly used to image vessels of the retina and conjunctiva. The purpose was to derive the best optical set-up for imaging vessels of the conjunctiva. METHODS: The concept of exposure density was used to predict a digital camera response to imaging vessels on a scleral background. Practical verification was performed to verify vessel contrast because of the difficulties in measuring the spectral components of the imaging system, such as the spectral reflectivity of vessels and sclera. Images of the same conjunctiva were repetitively taken through different coloured filters, using the Nikon FS-2 photo slit-lamp and recorded on different coloured channels of the Kodak DCS 100 digital camera. Gaussian blurred tubular models were fitted to densitometric profiles across three vessels from each image, allowing vessel contrast and width to be objectively measured. These measures were compared using different optical set-ups. RESULTS: Optimal exposure density calculations and vessel contrast was obtained with the xenon light source filtered with Wratten 99 (green) and Wratten 96 (neutral density, 0.2 log units) gelatine absorption filters using the green channel of the digital camera. This image set-up was associated with a 46% (99% CI 43-51%) to 64% (99% CI 58-72%) increase in contrast compared with vessels imaged without filtration, using the combined colour channel of the digital camera. Although differences in vessel widths resulted, absolute differences were marginal. CONCLUSION: With the increased use of digital imaging, and the need for image processing of vascular networks, image optimisation is beneficial. This study verified the optimal set-up for non-invasively imaging vessels of the bulbar conjunctiva.

 

Publication Types:

Validation Studies

 

PMID: 12090638 [PubMed - indexed for MEDLINE]

 

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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15005671

 

Ophthalmic Physiol Opt. 2004 Mar;24(2):74-81.  Related Articles, Links 

 

 

A comparison of manual and automated methods of measuring conjunctival vessel widths from photographic and digital images.

 

Owen CG, Ellis TJ, Woodward EG.

 

Department of Community Health Sciences, St George's Hospital Medical School, Cranmer Terrace, London SW17 ORE, UK. c.owen@sghms.ac.uk

 

We investigated the application of a fully automated computer algorithm for identifying vessels of the conjunctiva from their scleral surround, and compared measures of vessel width with established methods. Vessel widths at 101 locations (ranging from 20 to 140 micron), from 12 patients, were measured from film and digital images, using a variety of methods, and compared. Widths were measured manually, by semi-automated methods using grey level (densitometric) profiles taken from digital images, and by automated techniques set at different operating levels. Good intra-session repeatibility was obtained using the automated method with an operating sigma value of 3 pixels (16 micron) (mean difference 0.5 micron, 95% CI -8.5 to 9.4 micron) and manual calliper measurements from digitally created photographic slides (mean difference 0.4 micron, -9.3 to 10.1 micron). For comparison with other measures of width, the latter was used as the gold standard. Widths measured from film were slightly larger than those measured directly from digital images, although this effect was small (5 micron) for most vessels. Overall widths measured using the automated method, with a sigma value of 3 pixels, agreed best with the gold standard (inter-method repeatibility; mean difference 1.4 micron, -32.5 to 35.2 micron) although the automated method overestimated small widths (<40 micron) and underestimated larger vessel widths (>40 micron). Automated detection of vessels of the conjunctiva from digital images avoids manual and operator involved measures which are time consuming, and which preclude large patient studies. The resulting data may help in monitoring the vascular response of the conjunctiva to surgical or pharmacological intervention, and in describing vascular changes in response to ocular or systemic disease. The application of this algorithm to the study of retinal vessels is yet to be realised.

 

Publication Types:

Validation Studies

 

PMID: 15005671 [PubMed - indexed for MEDLINE]

 

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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=14756192

 

Int J Tissue React. 2003;25(3):105-15.  Related Articles, Links 

 

 

Microvascular assessment in Behcet disease: videocapillaroscopic study.

 

Pasqui AL, Pastorelli M, Puccetti L, Beerman U, Biagi F, Camarri A, Palazzuoli A, Servi M, Bischeri D, Saletti M, Bruni F, Auteri A.

 

Department of Internal Medicine and Immunology, University of Siena, Italy. pasquian@unisi.it

 

The aim of this study was to evaluate microvascular assessment in patients with Behcet disease (BD) by means of an intravital videocapillaroscopic study. Sixteen BD patients were compared with an equivalent group of healthy subjects matched for age and sex. Videocapillaroscopy (VCP) was performed in peripheral areas and in conjunctiva, and morphological and quantitative parameters were assessed. In both areas VCP showed several morphological alterations (microaneurysms, megacapillaries, desertification areas) detectable in a high percentage of patients; quantitatively we found significant changes of incisuring and sludging score, of capillary loop intermediate branch length (in peripheral areas) and of arteriole/venule diameter (in conjunctiva). Therefore, vessel involvement included both the number and the whole vessel structure and was seen both in peripheral and conjunctival areas when the two different vascular beds of micro- and paramicrocirculation were examined. We conclude that an important rearrangement of microcirculation is detectable in BD and that VCP may have diagnostic and prognostic value, providing qualitative and quantitative information able to define the systemic extension of vascular damage and the degree of vessel wall alteration.

 

PMID: 14756192 [PubMed - indexed for MEDLINE]

 

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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12897413

 

Clin Hemorheol Microcirc. 2003;28(4):221-7.  Related Articles, Links 

 

 

Online human conjunctival vessel diameter analysis. A clinical-methodical study.

 

Nagel E, Vilser W, Lanzl I.

 

e_a_nagel@t-online.de

 

BACKGROUND: The present study investigates the possible application of a commercially available on-line measuring device of retinal vessels for conjunctival vessel assessment. METHODS: Repeated measurements in one randomly chosen eye were performed in 11 healthy volunteers (mean age 42.9 +/- 10 years). Measurements of one conjunctival vessel were obtained first without a stimulus followed by measurements after the application of one drop of a topical vasoconstrictor. The examinations were performed by Retinal Vessel Analyzer (RVA, IMEDOS/Germany). This system determines automatically on-line the vessel diameter along a chosen vessel segment. RESULTS: Measurements in the native state without eye drop application showed an intraclass correlation coefficient of 0.97 and a mean variation coefficient of 1.8%. After application of the topical vasoconstrictor a short acting vasodilatation was observed with a magnitude of +10.9% +/- 14.9 (p < 0.001), followed by an increasing vasoconstriction (after 4 min -12.0% +/- 7.6; p = 0.004). One volunteer had no measurable conjunctival vessels in the baseline measurements and was therefore excluded from the study. DISCUSSION: The suggested technique allows the measurement of changes in conjunctival vessel diameter with high precision. The method represents a non invasive technique for the assessment of effects on conjunctival vessels caused by topical or systemic drugs.

 

Publication Types:

Evaluation Studies

 

PMID: 12897413 [PubMed - indexed for MEDLINE]

 

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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=14978131

 

J Immunol. 2004 Mar 1;172(5):3235-42.  Related Articles, Links 

 

 

Direct in vivo monitoring of acute allergic reactions in human conjunctiva.

 

Helinto M, Renkonen R, Tervo T, Vesaluoma M, Saaren-Seppala H, Haahtela T, Kirveskari J.

 

Department of Ophthalmology, Helsinki University Central Hospital Laboratory Diagnostics, Helsinki, Finland. maaret.helinto@helsinki.fi

 

Immediate allergic reactions are initiated by allergen-induced, specific IgE-mediated mast cell degranulation and involve leukocyte recruitment into the inflamed site. We compared conjunctival signs, symptoms, and in vivo leukocyte rolling and extravasation into sites of inflammation in five patients allergic to birch pollen and in 10 nonallergic controls who received a challenge to birch allergen or histamine. Both the specific allergen in allergic patients and histamine, both in patients and in healthy controls, induced symptoms and signs of an immediate allergic reaction together with leukocyte rolling within the conjunctival blood vessels. However, only allergen, not histamine, caused leukocyte extravasation into the site of inflammation in the allergic patients. Allergen also increased expression of endothelial P-selectin in conjunctival vessels and slowed the rolling of leukocytes which is required for their extravasation from blood circulation into the target tissue. Finally, i.v. heparin strongly reduced the number of slowly rolling cells during allergen- or histamine-induced reactions and this can probably hinder the leukocyte extravasation after allergen exposure. These findings suggest that slow rolling is required for leukocyte extravasation in acute allergic reactions, and it can be inhibited by heparin in vivo in therapeutically relevant conditions.

 

PMID: 14978131 [PubMed - indexed for MEDLINE]

 

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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11369629

 

http://www.bloodjournal.org/cgi/content/full/97/11/3401

 

Blood. 2001 Jun 1;97(11):3401-4.  Related Articles, Links 

 

 

Correlation of abnormal intracranial vessel velocity, measured by transcranial Doppler ultrasonography, with abnormal conjunctival vessel velocity, measured by computer-assisted intravital microscopy, in sickle cell disease.

 

Cheung AT, Harmatz P, Wun T, Chen PC, Larkin EC, Adams RJ, Vichinsky EP.

 

Department of Medical Pathology, University of California Davis School of Medicine, 4645 Second Ave., Sacramento, CA 95817, USA. atcheung@ucdavis.edu

 

The Stroke Prevention Trial has confirmed that utilization of transcranial Doppler ultrasonography (TCD), which examines blood flow in large intracranial vessels, can identify children with sickle cell disease (SCD) who are at high risk of developing a premature stroke. It is not known to what extent the vasculopathy in SCD involves small vessels and whether the abnormalities, if present, correlate with large-vessel vasculopathy. Eighteen children with SCD were examined with TCD to determine middle cerebral artery (MCA) velocity and computer-assisted intravital microscopy (CAIM) to determine bulbar conjunctival vessel velocity during the same visit for vasculopathy correlation. High MCA velocity (> or = 200 cm/sec) was found by TCD in 4 patients who also showed abnormal conjunctival velocity (< 0.2 mm/sec or intermittent trickle flow) by CAIM. Three patients had conditional (> or = 170 cm/sec and < 200 cm/sec) MCA velocity: 2 showed abnormal (trickle) and 1 showed normal conjunctival velocity (1.9 mm/sec). One patient with unmeasurable MCA velocity had abnormal (trickle) conjunctival velocity. Of the remaining 10 patients who had normal MCA velocity, 2 showed abnormal (0.05 mm/sec and 0.1 mm/sec) and 8 showed normal conjunctival velocities (1.1-2.4 mm/sec). The MCA velocities correlated significantly with bulbar conjunctival flow velocities (P < or =.008, Fisher exact test). A correlation exists between MCA (large-vessel) and conjunctival (small-vessel) flow velocities. CAIM is a noninvasive quantitative technique that might contribute to the identification of SCD patients at high risk of stroke. Small-vessel vasculopathy might be an important pathological indicator and should be further explored in a large-scale study. (Blood. 2001;97:3401-3404)

 

PMID: 11369629 [PubMed - indexed for MEDLINE]

 

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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10905796

 

Int Angiol. 2000 Jun;19(2):135-41.  Related Articles, Links 

 

 

Microvascular blood is distributed more to venules than to arterioles in patients with Buerger's disease. Observation of bulbar conjunctiva by intravital microscope system.

 

Homma S, Tsushima N, Minamiyama M, Hayashi T, Matsuo H.

 

Department of Internal Medicine, National Cardiovascular Center, Suita, Japan. homma-s@md.tsukuba.ac.jp

 

BACKGROUND: It has been a matter of controversy whether abnormalities of organs other than extremities may be a clinical manifestation of Buerger's disease (thromboangiitis obliterans; TAO). In the present investigation, our aim was to quantitatively characterise the configuration of microvascular networks in bulbar conjunctiva, which is not affected apparently, in patients with thromboangiitis obliterans. METHODS: Nine men with thromboangiitis obliterans attended our hospital and nine male volunteers as normal controls were enrolled in this study. We observed and analysed the configuration of the network of a bulbar conjunctiva by use of intravital microscope system with computer assisted image processing functions. Microvessel density was defined as a summation of vessel length in a ROI area and tortuosity was evaluated by a ratio of vessel length to direct distance of both terminals. RESULTS: In the microcirculation of bulbar conjunctiva in thromboangiitis obliterans, arteriole diameter was significantly decreased and density of venules was significantly increased. Increased venular density was mainly explained by increased tortuosity of venules. CONCLUSIONS: Consequently, microvascular blood was distributed more to venules than to arterioles in patients with thromboangiitis obliterans. Venule/arteriole ratios of diameter, tortuosity and microvessel density may be useful parameters to characterize the configuration of microvascular networks in thromboangiitis obliterans.

 

PMID: 10905796 [PubMed - indexed for MEDLINE]

 

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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9536887

 

http://bjo.bmjjournals.com/cgi/content/full/82/1/77

 

Br J Ophthalmol. 1998 Jan;82(1):77-81.  Related Articles, Links 

 

 

Capillaries in the epithelium of pterygium.

 

Seifert P, Sekundo W.

 

Alfried-Krupp-Laboratory, Department of Ophthalmology, University of Bonn, Germany.

 

AIM: To present new morphological observations of intraepithelial capillaries in pterygium and to provide some explanations for this phenomenon. METHODS: The ultrastructural features of pterygia from 26 patients were examined. Surgically excised tissue was processed for conventional light and transmission electron microscopy. RESULTS: Individual capillaries within the epithelium of the anterior half towards the head of pterygia were identified in 11 specimens out of 26 pterygia examined (42.3%). The perivascular connective tissue of the intraepithelial capillaries contained fibroblasts, collagen fibrils, and elastin-like material. Epithelial cells surrounding these capillaries showed defects in the basal lamina in contrast with the continuous basal lamina of the endothelium. In the intercellular space of the epithelium an amorphous substance, occasional fibroblast processes, and collagen fibrils were frequently observed. CONCLUSION: Capillaries in the epithelium of pterygia are rare, but not exceptional. The ingrowth of these vessels from the stroma into the epithelium can be interpreted as a reaction to hypoxia or deficiency of any other substance transported via the bloodstream. Apparently, the perivascular connective tissue can be used by ingrowing fibroblasts as a migration pathway. The migrating fibroblasts appear to use the defects of the epithelial basal lamina (whether partially or complete) in order to reach the intercellular space. It is possible that collagen fibrils in the epithelial intercellular space have been laid down by fibroblasts which contribute to the pathological dedifferentiation of the conjunctival epithelium.

 

PMID: 9536887 [PubMed - indexed for MEDLINE]

 

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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1494054

 

J Mal Vasc. 1992;17(4):273-6.  Related Articles, Links 

 

 

Raynaud's features in childhood. Clinical, immunological and capillaroscopic study.

 

Navon P, Yarom A, Davis E.

 

Department of Pediatrics, Bikur-Cholim General Hospital, Jerusalem, Israel.

 

Raynaud's phenomenon, uncommon in childhood, often heralds connective tissue disorder. Since microvascular abnormalities can be detected at an early stage of the connective tissue disease, especially in scleroderma, a specific diagnosis can be made in patients presenting with Raynaud's phenomenon alone or Raynaud's phenomenon associated with symptoms suggestive of connective tissue disease. Raynaud's phenomenon was studied in 11 consecutive children, 10 girls and 1 boy, ages 6 to 15. One child had a definite diagnosis of cutaneous polyarteritis nodosa. In 6 others connective tissue disease was suspected: 4 had arthritis, 2 has telangiectasia, leg ulcers and antinuclear antibodies. Of the remaining 4, one had hemiplegia and 3 Raynaud's phenomenon only. Oscillometry of the radial artery was reduced in 7 of 9. Decreased capillary resistance was found in 2 of 6, while abrupt thinning in conjunctival vessels was seen in 3 of 7. On nailfold capillaroscopy, reduced vascularity was noted in 5 of 11, dilated capillaries in 4 of 11, tortuousity in 2 of 11, capillary thinning in 1 of 11, capillary spasm in 1 of 11 and normal pattern in 3 of 11. Two patients presenting with Raynaud's phenomenon were found to have "scleroderma-like pattern" on nailfold capillaroscopy. One of them died 2 years later of cardiopulmonary sclerosis, and another developed esophageal stricture and Barrett's esophagus. Neither has sclerodermatous skin. In childhood Raynaud's phenomenon, nailfold capillaroscopy is a non-invasive examination enabling early diagnosis of "systemic scleroderma sine scleroderma".

 

PMID: 1494054 [PubMed - indexed for MEDLINE]

 

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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1860387

 

Zhonghua Shen Jing Jing Shen Ke Za Zhi. 1991 Apr;24(2):84-6, 124.  Related Articles, Links 

 

 

[Bulbar conjunctival microcirculation in schizophrenics]

 

[Article in Chinese]

 

Xie Y, Wu L.

 

Jiangxi province Mental Hospital Nanchang.

 

A comparative observation on bulbar conjunctival microcirculation (BCM) between 38 schizophrenics and 38 normal controls was carried out. The results showed 95% abnormal BCM in schizophrenics. And among them, the moderate and severe abnormal BCM was 74%. And in all of the 14 investigated items, the general abnormalities were 50%. But in normal controls, abnormal BCM was 27%, and just a few of them appeared the general abnormalities, and no one suggested moderate and severe abnormal BCM. As compared the results obtained in schizophrenics with those in normal controls, we found 12 investigated items such as irregularity in diameter, dilatation and tortuosity of tiny vena; irregularity in diameter, stiff of tiny arteria; small hemorrhagic spot, brown pigmentation, red blood cells collected in tiny vena and capillary, small vascular density, speed of blood flow and arterio-venous ratio were statistically significant.

 

PMID: 1860387 [PubMed - indexed for MEDLINE]

 

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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2301526

 

Am J Ophthalmol. 1990 Feb 15;109(2):153-61.  Related Articles, Links 

 

 

Hemorheologic abnormalities in patients with human immunodeficiency virus infection and ophthalmic microvasculopathy.

 

Engstrom RE Jr, Holland GN, Hardy WD, Meiselman HJ.

 

Jules Stein Eye Institute, UCLA Medical Center 90024-1771.

 

The severity of conjunctival microvascular changes and the presence of cotton-wool spots were compared to factors that may affect blood flow (hematocrit level, red cell aggregation, fibrinogen level, plasma viscosity, circulating immune complexes, and quantitative immunoglobulin levels) in 22 human immunodeficiency virus-infected individuals. The severity of conjunctival disease was associated with increased zeta sedimentation ratios (a measure of red cell aggregation) and fibrinogen levels. The presence of cotton-wool spots was also associated with higher fibrinogen levels. Plasma viscosity and quantitative IgG levels were above normal levels in most patients, although a relationship to disease severity was not established. Altered blood flow may contribute to vascular damage and ocular ischemic lesions in patients with human immunodeficiency virus infection.

 

PMID: 2301526 [PubMed - indexed for MEDLINE]

 

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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2118030

 

Chin Med J (Engl). 1990 Feb;103(2):134-41.  Related Articles, Links 

 

 

A family with Fabry's disease. Ocular manifestations and transmission electron microscopic examination of a skin lesion biopsy.

 

Bao LL, Guo LL, Li SN, Xiao J, Yang JS, Bai LR, Ye PM, Guo ZT, Liu DW.

 

Shanxi Medical College, Taiyuan.

 

A family with Fabry's disease including 2 hemizygotes and 3 heterozygotes is reported. The ocular manifestations include tortuosity of conjunctival vessels, Fabry's deposits underlying the anterior capsule of the lens and the whorl-like corneal dystrophy. Foam cells or mulberry cells in the urinary sediment and varying numbers of high electron-dense inclusions in the cytoplasm of endothelial cells in skin lesions are also important in diagnosing this disease. The concentrations of lipid peroxide in the sera of 2 hemizygotes in this report were higher than normal, possibly due to the patients weak ability of anti-oxygenation and malfunction of cells whose plasma membranes are easily attacked by free oxygen radicals. The biochemical and pathological changes, diagnosis, treatment, genetics and prevention of the disease are discussed.

 

PMID: 2118030 [PubMed - indexed for MEDLINE]

 

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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3654069

 

Int J Microcirc Clin Exp. 1987 Aug;6(3):245-55.  Related Articles, Links 

 

 

Analysis of microvascular network in bulbar conjunctiva by image processing.

 

Chen PC, Kovalcheck SW, Zweifach BW.

 

AMES-Bioengineering, University of California, San Diego, La Jolla 92093.

 

A digital image processing procedure has been developed for obtaining quantitative morphometric data on the microcirculatory network in the human bulbar conjunctiva. Highlights of this semi-automated approach include: 1. extraction of morphometric information that cannot be readily obtained by manual methods--length, diameter, and diffusion distributions; 2. speed and consistency in data generation--only 10 minutes are required to scan and to analyze 6.7 mm2 of conjunctiva microvasculature. The variation was less than 5% when images of the same area in the same eye were subjected to analysis at different times; 3. avoid the human bias factor--the data obtained by repeated analysis of the same negative varied by less than 0.02%.

 

PMID: 3654069 [PubMed - indexed for MEDLINE]

 

 

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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3814565

 

Br J Ophthalmol. 1987 Jan;71(1):2-10.  Related Articles, Links 

 

 

Low dose fluorescein angiography of the conjunctiva and episclera.

 

Meyer PA, Watson PG.

 

By reducing the dose of injected fluorescein its leakage from conjunctival and episcleral capillaries has been minimised. These vessels have been demonstrated with great clarity, and the venous circulation, previously obscured by extravascular fluorescein, has also been revealed. The anatomy of the anterior segment vessels, and the blood flow within them, has been studied in eight normal subjects. The anterior ciliary arteries feed an anterior episcleral arterial circle that has superficial and deep components. This supplies the anterior conjunctival and episcleral circulations, the limbal arcades, and the iris arterioles. Where the superficial arterial circle is deficient, isolated vessels emerge from the deep segments of the circle to supply the episcleral plexus and conjunctival arterioles. Watershed zones between the anterior and posterior territories of the conjunctival and episcleral circulations overlap. They may fluoresce up to 30 seconds after the anterior ciliary arteries. The scope of this technique and the implications of these findings are discussed.

 

PMID: 3814565 [PubMed - indexed for MEDLINE]

 

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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3590013

 

Ter Arkh. 1987;59(4):97-100.  Related Articles, Links 

 

 

[The use of thermography in the clinical evaluation of microcirculation]

 

[Article in Russian]

 

Iakhontova OI, Rutgaizer IaM, Somova EP.

 

A method of thermography of the hands including the cold test was employed to evaluate peripheral microcirculation in 116 patients (34 with atherosclerosis of the vessels of different sites, 38 with chronic diffuse liver diseases and 38 with chronic pancreatitis). In 29 patients with hepatocirrhosis and chronic pancreatitis microcirculation was also studied by conjunctival biomicroscopy. Microcirculatory disturbances by the method of thermography of the hands, particularly using the cold test, were revealed in a considerable number of cases of primary vascular pathology as well as in chronic liver and pancreatic diseases. Parallelism in the recognition of microcirculatory disturbances by the methods of thermography and conjunctival biomicroscopy was noted. The method of thermography of the hands permitted the evaluation of reactivity of the vessels of the microcirculatory bed. Taking into account its sufficient informative value, noninvasiveness, simplicity and an opportunity of dynamic observations it can be recommended for a wider clinical use to study microcirculatory disturbances.

 

PMID: 3590013 [PubMed - indexed for MEDLINE]

 

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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3784446

 

Klin Wochenschr. 1986 Oct 1;64(19):953-5.  Related Articles, Links 

 

 

Microcirculation in the conjunctival capillaries of healthy and hypertensive patients.

 

Korber N, Jung F, Kiesewetter H, Wolf S, Prunte C, Reim M.

 

To study the impact of hypertension on conjunctival circulation, one group of healthy individuals and three groups of hypertensive patients (different stages of disease) were examined. Each participant had to undergo videomicroscopy to determine erythrocyte velocity in conjunctival capillaries, reactive hyperaemia and the diameter of the erythrocyte column within the vessels. Compared to healthy subjects, erythrocyte velocity and the diameter of the erythrocyte column were markedly increased in hypertensive patients. Furthermore the normalization of the erythrocyte velocity after a period of hypoxia was impaired in the hypertensives. These results indicate that changes in microcirculation due to hypertension can be detected by video microscopy.

 

PMID: 3784446 [PubMed - indexed for MEDLINE]

 

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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3786297

 

Probl Endokrinol (Mosk). 1986 Sep-Oct;32(5):18-22.  Related Articles, Links 

 

 

[Microcirculatory disorder in the bulbar conjunctiva of patients with diabetic retinopathy]

 

[Article in Russian]

 

Danilova AI.

 

Biomicroscopy was employed to study the state of microcirculation of the bulbar conjunctiva in 166 patients with diabetes mellitus at different stages of the ophthalmoscopic assessment of the fundus of the eye. Disorder in conjunctival microhemodynamics was found in 89.7% of the patients, in the presence of retinopathy in all the cases and in a normal picture of the fundus of the eye in 69.3%. Qualitative and quantitative changes in microcirculation of the conjunctiva were shown to depend on the stage and form of diabetic retinopathies. Values of changes of the total conjunctival index and its constituents were on an increase with aggravation of a pathological process in the fundus of the eye. Vascular changes prevailed in the diabetic-sclerotic and diabetic-hypertensive forms of retinopathies, intravascular changes and perivascular edema prevailed in the diabetic-renal form.

 

PMID: 3786297 [PubMed - indexed for MEDLINE]

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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3831149

 

Int Angiol. 1985 Jul-Sep;4(3):275-83.  Related Articles, Links 

 

 

Microcirculation in the elderly.

 

Tassi G, Maggi G, de Nicola P.

 

Studies on microcirculation in the elderly include observations in the small vessels of the bulbar conjunctiva and of the nailbed with respect to a classification of the findings according to a number of semeiologic criteria (diameter and shape alterations, terminal capillary network, intravascular red cell aggregation). In vascular diseases of the elderly there are typical alterations of the capillaroscopic findings in the bulbar conjunctiva and in the nailbed, particularly in cases of arteriosclerosis, arterial hypertension, diabetic microangiopathy, heart failure, ischemic myocardiopathies. During the treatment with some vasoactive drugs (nicotinic acid and its derivatives, buflomedil, CPD-choline) there are marked modifications of the small conjunctival vessels, with evident dilatations, appearance of collaterals, increased homogeneity of the blood flow, better evidence of the capillary network and reduction of intravascular red cell aggregation.

 

PMID: 3831149 [PubMed - indexed for MEDLINE]

 

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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2579512

 

ZFA. 1985 Jan-Feb;40(1):51-5.  Related Articles, Links 

 

 

[Aging changes in conjunctival blood vessels]

 

[Article in German]

 

Siprova H, Vacek L.

 

The relation of the changes in the conjunctival blood vessels to the age were studied in a group of 217 persons aged 20 to 79 years. All persons suffering from arterial hypertension, diabetes, systemic diseases and manifested forms of arteriosclerosis were excluded. It has been proved that examination of the conjunctival blood vessels by means of the slot-lamp facilitates the study of the changes in the microscopic blood vessels in relation to the age. Most conspicuous was the increase of the meander course of the blood vessels, also the increasing number of blood vessels is more frequent in the group of older persons. Clusters of vessels and a narrowing of the diameter of the arterioles was seen only in persons over fifty years of age. The "upright" course of the arterioles was found only in individual cases and without any special dependence to age. Sacculations on the small veins and microaneurysms on the arterioles were not observed in any of the examined group of healthy persons.

 

PMID: 2579512 [PubMed - indexed for MEDLINE]

 

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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6707531

 

J Mal Vasc. 1984;9(1):23-8.  Related Articles, Links 

 

 

[Capillaroscopy and Raynaud's phenomenon]

 

[Article in French]

 

Carpentier P, Franco A.

 

Nailfold capillary microscopy is a simple and noninvasive screening test for the etiological diagnosis of Raynaud's phenomenon: --dilating capillary dystrophia, with its large capillary loops (megacapillaries), it is a very specific sign of connective tissue disorders, especially systemic sclerosis; --meshed or branched-tree shaped capillaries are neocapillaries, the meaning of which is very often a dermal vasculitis (S. L. E., rheumatoid arthritis, paraneoplastic Raynaud's phenomenon, or even chilblains); --when numerous abnormal capillary loops and hemorrhages are seen without any special pattern, the diagnosis of idiopathic Raynaud's disease is very unlikely and an extensive biological investigation is needed; --nailfold capillaries look like normal in idiopathic Raynaud's disease and, in this case, only a sharp physical examination and a few biological tests are required. Conjunctival angioscopy is a useful complementary test in some cases when nailfold capillaroscopy is unreliable. Capillary video microscopy is an interesting method for further investigations about the physiopathology of Raynaud's phenomenon.

 

PMID: 6707531 [PubMed - indexed for MEDLINE]

 

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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7129189

 

Gig Tr Prof Zabol. 1982 Aug;(8):28-33.  Related Articles, Links 

 

 

[Use of conjunctival biomicroscopy for the early diagnosis and assessment of the effectiveness of pathogenetic therapy of chronic chromium poisoning]

 

[Article in Russian]

 

Iakovlev NA, Kosherov KB, Sliusar' TA, Bralov KB.

 

PMID: 7129189 [PubMed - indexed for MEDLINE]

 

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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7093046

 

Biull Vsesoiuznogo Kardiol <